Product description
γ-Glutamylcysteine synthetase (γ-GCS, EC 6.3.2.2) is the rate-limiting enzyme in glutathione (GSH) synthesis, catalyzing the synthesis of γ-glutamylcysteine (γ-GC) from glutamate and cysteine. Studies have shown that this enzyme plays a role in cellular oxidative stress. In the presence of ATP and magnesium ions, γ-GCS catalyzes the synthesis of γ-glutamylcysteine (γ-GC) from glutamate and cysteine, while ATP dephosphorylates to produce inorganic phosphate molecules. The activity of γ-GCS can be calculated by measuring the rate of increase of inorganic phosphate.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.