Affinity- Purification /mass Spectrometry (AP-MS) Service
Affinity- Purification /mass spectrometry (AP-MS) is a mainstream experimental method for identifying Protein protein interaction (PPIs) in a high-throughput manner and it has been greatly improved in the background of the improvement of sample enrichment and separation chromatography methods, as well as the improvement of the resolution and sensitivity of mass spectrometry analysis. This approach has been and continues to be instrumental in identifying the interactor proteins.
Figure 1 Affinity purification and mass spectometry (AP-MS).
AP-MS is now the standard method for discovering protein-protein interactions. Compared to traditional PPI studies, AP-MS does not require the purchase or development of specific antibodies for each new decoy protein, but instead uses epitope tags or capture probes to perform similar high-throughput screening.
Technical advantages of AP-MS:
- The interaction protein obtained is the natural interaction between the bait protein and the cell, which conforms to the real physiological situation in the body and has high reliability;
- Do not use antibody "pull" interaction protein complex, avoid antibody pollution;
- The eluent obtained can be directly enzymatically hydrolyzed by mass spectrometry to reduce the protein loss caused by running glue.
We provide you with the following AP-MS services:
The customer shall provide the name, sequence, species and other relevant information of the bait gene or the cDNA template of the bait gene, as well as the cells to be studied (ensure that the cells are in good condition and easy to be transfected with the plasmid) and the information related to culture
Our service results will provide you with the constructed eukaryotic expression vector, namely the protein expression verification results, the experimental group and control group protein identification results, bioinformatics analysis results, etc.
Profacgen is an international company with international customers, whose trust and respect are vital to our technology and product sales. We will do our best to protect your technology and commercialize it for you.
If you want to know more test information and price information, please feel free to contact us and we will reply to you as soon as possible.