Stable Cell Line Construction for Protein Expression

Cell line development is the process of creating a large population of identical cells that contain the gene that, on translation, encodes the biologics (recombinant proteins, antibodies, fusion proteins, vaccines, etc.). Chinese hamster ovary (CHO) cells and human embryonic kidney (HEK) cells are among the most efficient and prolific biofactories, and their cultures are widely used to produce novel cell lines.

When large quantities of recombinant protein are required, the construction of stable expression lines is recommended for reproducible and cost-effective protein production. Stable cell lines can be used for long-term continuous growth without significant changes in the expression levels of the target gene. It is widely used in functional studies of genes, protein production and drug screening, for both therapeutic and non-therapeutic purposes. Construction of stable cell lines usually takes 3 steps: delivering the target gene into host cells, screening the stable expression clones, and stability testing.

Profacgen provides stable cell line construction services both for research use and industrial production. Our cell lines support μg to g scale protein expression levels and are continuously optimized to meet the specific requirements of therapeutic cell line development as well as diagnostic and research reagents.

• Antibody
• enzyme
• fusion protein
• bispecific
• biosimilars
• cytokine
• hormones
• growth factor

Our Services

At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve a high integration rate. Limited dilution and antibiotics screening are then applied to select high-yield, genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein, we can also use flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of your target gene. Doxycycline can then be used to induce the expression of your target gene. We perform quality control of cell lines through batch culture, mycoplasma detection, identification and freezing of stable producers.

To ensure the integration of your target gene and stable/inducible expression of the protein, we can provide PCR, qRT-PCR, microscopy, flow cytometry analysis and stability test according to your specific requests.

Profacgen has accumulated lots of experience in stable cell line construction for protein expression. Our experienced scientists, well-validated cell line platforms and state-of-the-art facilities guarantee our high-quality services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.