Surface Plasmon Resonance (SPR) Service
Profacgen provides a comprehensive panel of services for the study of protein-protein interactions, of which the Surface Plasmon Resonance (SPR) analysis is commonly used by our customers for the highly accurate and sensitive kinetics and affinity measurement. For SPR analysis, the ligand-of-interest is immobilized on the surface of the biosensor chip, while the analyte is injected to flow through the chip surface. Dynamic change of the SPR angle can be detected and assayed for binding affinity when interaction happens between the ligand and the analyte.
The SPR technique is based on a complex optical phenomenon. When photon reaches the interface of two medium with different refractive indexes at a specific angle range, the resonant oscillation of surface plasmon generated by metal free electron can be induced. The resonance also requires that the frequency of incident photon matches the natural oscillating frequency of the surface plasmon. Once the resonance happens, the energy of the reflected photon will be absorbed and the light dramatically weakened. Within the SPR angle range, the SPR angle (or resonance angle) is defined as the angle at which nearly no reflect photon can be detected. Since the introduction of SPR into the immune sensor field by Liedberg, Nylander and Lundström in 1983, surface plasmon optical biosensor has been extensively studied and widely used. Later on, the SPR technology was further developed to study the interaction between ligand and analyte on a biosensor chip. As the SPR angle changes with surface refractive indexes, which is in direct proportion to the molecular mass of the biomolecule attached to the metal surface, the binding of the analyte can be detected at high sensitivity.
Principle of the SPR Technology [1]
Profacgen provides professional SPR analysis service. Our service has been extensively applied to determining the interaction between proteins, peptides, oligonucleotides, bacteria, cells and small molecular compounds.
Advantages of the SPR technology:
Sample requires no labeling or modification before analysisLow requirement on sample purity
In situ, real time dynamics study under natural conditions
High throughput and high sensitivity analysis
Ease of manipulation
Requires only small amount of sample
Our strengths:
Advanced equipment and technique
Experienced scientific team
Highly reliable and reproducible result
Short turnaround time
Best price in the market
Please feel free contact us for more information of our SPR service.
References:
[1] Chang L, Tingjun L, Kosuke I, et al. Real-time monitoring biomarker expression of carcinoma cells by surface plasmon resonance biosensors[J]. Chem Commun, 2012, 48(84): 10389-91.