Nuclear Magnetic Resonance (NMR)

The functioning of cells depends on the ability of communication networks of cell societies to transmit essential information as signaling pathways, and these cellular responses are mediated by numerous biomolecular interactions. Including cancer, chronic inflammatory syndromes and diabetes, all result from the malfunction of one or more steps in the signaling pathway. Therefore, the atomic-resolution exploration of protein-protein interactions is critical for developing effective strategies for therapeutic interventions against human diseases.

We introduce nuclear magnetic resonance spectroscopy (NMR), the most common technique for studying protein-protein interactions. NMR provides atomic resolution information about binding interfaces, intermolecular affinity, and binding-induced conformational changes, etc. Notably, continued advancements in solution and solid-state NMR techniques have established this method as a fundamental research tool to accelerate our biochemical research of signal transduction pathways.

Figure 1. nuclear magnetic resonance spectroscopy for protein research (Cai, K.; Markley, J. 2018)

NMR service provided by Profacgen:

Profacgen scientists will provide high-quality NMR spectroscopy, including 300 MHz, 400 MHz and 500 MHz NMR, taking advantage of this powerful tool to provide professional assistance in all types of biochemical research. Customers can perform many studies of interest on our comprehensive NMR platform and quickly obtain accurate analytical reports. The NMR services we provide include but are not limited to:

• Quantitative Nuclear Magnetic Resonance (qNMR) Analysis
• Solid State Nuclear Magnetic Resonance Analysis
• Solution State Nuclear Magnetic Resonance Analysis

Applications:

• Interaction research
• Polymer characterization
• Characterization of chemical structures of liquids and solids
• Mixture component quantification
• Drug development

As a protein research expert, Profacgen has accumulated a lot of successful experience in protein interaction analysis, and has assisted many customers around the world to complete projects with high efficiency and high quality. Our highly competitive prices and unprecedented expertise have earned us widespread acclaim. Request more information to find out how Profacgen can help.

References

1. Cai, K.; Markley, J. NMR as a Tool to Investigate the Processes of Mitochondrial and Cytosolic Iron-Sulfur Cluster Biosynthesis. Molecules. 2018.
2. Purslow, J.A.; et al. NMR Methods for Structural Characterization of Protein-Protein Complexes. Front. Mol. Biosci. 2020.