Lactic Acid Bacteria System Protein Expression Service

Background

Lactic acid bacteria (LAB) are a type of anaerobic bacteria collectively known for their ability to utilize fermentable carbohydrates to produce large amounts of lactic acid. These bacteria are widely distributed in nature, exhibiting rich species diversity with at least 18 genera and over 200 species. LAB are important probiotics, holding significant positions in the food industry and demonstrating immense potential in biomedicine. LAB system protein expression technology has emerged as a hot research topic in recent years. As a homotypic fermentation bacterium, LAB can swiftly convert lactose into lactic acid. Their functional characteristics, such as carbon metabolism akin to cocci, extracellular and intracellular protein hydrolysis systems, antibiotic production, phage interactions, and resistance, have been extensively studied, facilitating wide-ranging applications of LAB in biotechnology.

LAB system protein expression expands the application scope of genetic engineering technology and introduces new ideas and methods for biomedicine. This service enables the production of specific functional protein drugs for treating intestinal diseases, immune system disorders, and more. Furthermore, LAB system protein expression technology can be utilized to produce food additives, dietary supplements, etc., offering more choices for people's health and well-being.

Profacgen has independently developed a Lactic Acid Bacteria protein expression platform capable of offering various expression schemes tailored to customer needs, including heat-inducible expression, food-grade expression, and secretion-type expression. We strive to provide high-clarity, high-purity, and high-activity LAB protein expression services. Through genetic engineering techniques, we introduce target genes into LAB to achieve the expression and secretion of exogenous proteins. LAB system protein expression technology is advantageous for its safety, efficiency, and cost-effectiveness.

Service Procedure

Profacgen provides customers with one-stop lactobacillus system protein expression services, ensuring meticulous planning and rigorous quality control at every stage, from gene cloning to protein expression. Below is our detailed service process designed to offer comprehensive support for customers' research or production projects:

1. Project Consultation and Confirmation
   Our professional team will first communicate thoroughly with you to understand your specific needs, goals, and expectations. Based on this information, we will provide personalized project consultation and design a plan to ensure our services fully meet your requirements.
2. Gene Cloning and Vector Construction
   During the gene cloning phase, we will optimize codons for your sequences and employ advanced gene cloning techniques to accurately and efficiently integrate the target gene into the vector.
3. Small-Scale Protein Expression Analysis and Identification
   Electroporation will introduce The constructed expression vector into lactobacillus host cells, followed by small-scale expression analysis and identification. We will optimize culture conditions for lactobacillus, such as temperature, pH, and nutrient composition, to induce the expression of the target protein.
4. Protein Expression Purification
   The expressed lactobacillus will undergo purification using techniques such as centrifugation, filtration, and chromatography to ensure the purity and activity of the final product.
5. Quality Testing
   To ensure the quality and purity of the protein product, we employ various methods, including but not limited to gel electrophoresis, HPLC analysis, and Western blot detection, to verify that the protein's function and purity meet requirements.
6. Product Delivery
   Upon successful quality testing of the protein product, we will package and deliver the qualified protein product in freeze-dried form according to your specifications and timeline.

Our Advantages

• Multiple optional expression modes
  Standard system: PNZ8148 standard vector + NZ9000 standard strain;
  Food-grade expression system: PNZ8149 food-grade vector + NZ3000/3900 food-grade strainEfficient expression optimization
• One-stop service
  We offer a one-stop lactobacillus system protein expression service, from demand consultation to final delivery, providing full-process tracking to ensure customers obtain the target protein they need.
• High cost-effectiveness
  We are committed to providing cost-effective services, combining excellent quality with affordable prices, and ensuring that customers enjoy a great value-for-money experience.

Case Study

Lactobacillus protein expression service vector

Restriction digestion verification

Protein expression identification

FAQs