A New Platform for Protein High-Throughput Screening

High-throughput screening refers to the method of automatically testing a large number of chemical or biological compounds to find specific biological targets, which has become one of the essential technical means for discovering innovative drugs. High-throughput is a new technology system for target screening based on molecular and cellular experiments, assisted by automation technology. It integrates multiple disciplines such as chemistry, genomics, bioinformatics, and automation. The computer analyzes and processes thousands of sample data to obtain scientifically-accurate experimental results.

With the development of science and technology, the methods of high-throughput screening technology are also constantly optimized. Among the various techniques of high-throughput detection, reporter gene-based detection is the most widely used technology, and it has gradually become the main detection method in drug discovery. However, since the small molecule compounds contained in the high-throughput screening library often interact with the reporter protein, this leads to the generation of a large number of false-positive signals during the screening process, resulting in the need for further detection of the screening results, which will cause time waste and expense costs increase.

Recently, researchers have developed a new platform for high-throughput detection of proteins to optimize false positives. The platform can effectively distinguish the signal generated by the detection target from the false-positive signal generated by the reported gene, which significantly improves the reliability of the detection result. The platform performs multiple validations of the detection results by introducing multiple reporter genes of different origins. And this new platform achieves ribosome skipping translation by submitting short peptides in multiple reporter genes, thus forming an easily detectable "circuit". Only signals from responses to different reporter genes can prove that the result is a true response signal in this screening platform. By optimizing high-throughput screening methods, this platform effectively improves the accuracy of screening results, showing great potential in the discovery and development of new drugs.

The Advantages of this platform include:

• Reduce the false positive probability
• Short detection time
• High reproducibility of results

Potential Applications

• Protein High-throughput screening
• Drug discovery and development
• High-throughput library screening

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Please do not hesitate to contact us for more details if you are interested in this new method, and we will provide considerate service for you. At the same time, we also offer other services and biomarker products; please move to our website for more details.

Reference

1. Mercola M, Colas A, Willems E. Induced pluripotent stem cells in cardiovascular drug discovery. Circ Res. 2013 Feb 1;112(3):534-48. DOI: 10.1161/CIRCRESAHA.111.250266.