Enzyme-linked Immunosorbent Assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA), first described by Weiland in 1978, is a versatile and validated detection technique commonly used to detect peptides, proteins, antibodies, glycoproteins, and hormones in biological samples and is the gold standard for protein quantification in ligand binding assays. This technique allows researchers to perform antibody-based quantitative measurements on any target with sensitive and specific binding reagents such as antibodies or aptamers. Typically, an enzyme, such as horseradish peroxidase, is directly or indirectly conjugated to the antibody to provide a detection signal. The choice of antibody and detection method will determine the sensitivity and specificity of the detection. This biochemical technique has been widely used as a diagnostic tool in medicine, pathology, and biotechnology, as well as quality control in various industries.

In direct ELISA assays, antigens are immobilized in multiwell plates by adsorption. The enzyme-linked detection antibody is then added and incubated with the antigens. In the final step, the enzyme substrates are added to the wells and conjugated enzyme activity is assessed by incubating with the substrates to produce a visible signal that correlates with the amount of antigen. In addition to direct ELISA, there are many more complex ELISA methods, such as indirect ELISA, sandwich ELISA, competitive ELISA, etc.

Figure 1. Schematic presentation of basic types of ELISA (Boguszewska, K.; et al. 2019)

Services

As a protein research expert, Profacgen has accumulated a lot of successful experience in protein functional analysis and characterization testing. Our first-class equipment and experienced scientists can provide customers with enzyme-linked immunosorbent assay (ELISA) efficiently and at low cost.

Our procedure ensures highly specific antibody-antigen binding, greatly improving the quality of experiments. In addition to satisfying customers with multi-purpose ELISA services, including direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA services, we also customize the optimal solution for customers who require validation/production kit for projects. Depending on the required sensitivity and technology, we can develop colorimetric assays, fluorometric assays, luminescent assays, and MSD, among others.

Profacgen has accumulated lots of experience in enzyme-linked immunosorbent assay. Our professional technical team can provide customers with high-quality direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.

Reference

1. Boguszewska, K.; et al. Review: immunoassays in DNA damage and instability detection. Cellular and Molecular Life Sciences. 2019.