Bacterial Expression

Bacteria are the most widely used protein expression systems in which fast production of recombinant proteins has been achieved due to their rapid growth, high yields, simple culture conditions and ease of scale-up. Bacterial expression systems also feature low production costs and high scalability, making them an attractive option for recombinant protein production.

The most widely used bacterial host is E. coli. It is the most established tool and is almost always the first choice if applicable. High-efficiency expression and production of various recombinant proteins such as cytokines, enzymes, immunogens and antibody fragments have been performed by E. coli. Another bacterium, Bacillus subtilis, is an increasingly popular host for protein expression and is an FDA-designated Generally Recognized As Safe (GRAS) organism. It is a Gram-positive bacterium, which makes this system free of endotoxin concern. It also has much less codon bias than E. coli. Furthermore, it secretes protein directly into the culture media, which highly simplifies subsequent protein purification steps. All the above features make it an attractive system for the production of soluble and properly folded recombinant proteins at both laboratory and industrial levels.

Our Services

Profacgen offers you the ultimate solution for bacterial production. Our services can be tailored to your specialized requirements (codon optimization, tag selection, purity, endotoxin removal, protein refolding, etc.) to best match your downstream applications. Our expertise guarantees high protein quality, short turnaround times and the best prices in the market.

Our Service Process

• Gene synthesis and plasmid preparation: Codon optimization, subcloning, DNA QC.
• Protein expression evaluation and optimization: Use SDS-PAGE and Western blot to confirm expression levels and optimize protein expression conditions.
• Scale-up and protein purification: Scale up protein expression using optimized conditions, criteria: protein purity >90%, endotoxin <1.0 EU/μg.

Our Featured Services

• Protein Labeling: Various protein labeling techniques, including isotopic labeling, biotinylation, and HRP conjugates.
• Endotoxin Removal: Accurate endotoxin detection and removal services with detection levels below 1.0 EU/μg.
• Inclusion Body Refolding: Inclusion body refolding condition screening, IB treatment, optimization of purification and analytical methods.

See more details on the principle and protocol of bacteria expression system on our website. See more...

Profacgen has accumulated lots of experience in protein expression. Our professional technical team can provide customers with high-quality bacterial expression systems and many related featured services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.