Anaerobic Bacteria Protein Expression Service

Background

With the rapid development of biotechnology, protein expression services have become an indispensable part of life science research. Among various protein expression systems, anaerobic bacterial systems have attracted considerable attention due to their unique biological characteristics and potential applications. Anaerobic bacteria are a group of microorganisms that grow in anaerobic or microaerophilic environments. They utilize unique metabolic pathways, such as anaerobic fermentation of proteins, to convert organic compounds into energy and metabolic products. Anaerobic bacterial systems protein expression services are based on this principle, employing anaerobic bacteria as host cells. Through genetic engineering techniques, foreign genes are introduced into these bacteria to achieve efficient expression of target proteins.

The protein expression service using anaerobic bacterial systems offers significant advantages. Firstly, anaerobic bacteria have a well-defined genetic background, allowing researchers to predict and manipulate their gene expression processes more accurately. This clear genetic background helps reduce experiment uncertainties, thereby enhancing the reliability and reproducibility of experiments. Secondly, anaerobic bacterial systems often yield higher levels of protein expression. This is particularly crucial for studies and applications requiring large-scale production of target proteins. By optimizing expression conditions, protein yields can be further increased to meet experimental and production needs. Additionally, proteins expressed using anaerobic bacterial systems typically exhibit good stability. This stability ensures that the protein retains its activity for an extended period after expression, minimizing losses during subsequent purification and storage processes. Moreover, stable proteins are easier to analyze and utilize in downstream applications.

Profacgen offers high-quality anaerobic bacterial systems protein expression services for producing essential proteins such as enzymes, antibodies, hormones, etc., providing strong support for clients' drug development endeavors.

Service Procedure

The process of anaerobic bacterial systems protein expression services mainly includes steps such as gene cloning, expression vector construction, transformation and screening, expression condition optimization, and protein purification. Profacgen offers multiple optimized steps to ensure efficient expression of the target protein:

1. Gene Cloning:
   Profacgen optimizes the gene sequence codons according to the expression system and uses appropriate restriction endonucleases and ligases to insert them into suitable expression vectors.
2. Expression Vector Construction:
   Profacgen selects suitable promoters, terminators, ribosome binding sites, and antibiotic resistance genes for screening based on anaerobic bacterial expression, optimizing transcription and translation efficiency of the target gene.
3. Transformation and Screening:
   Profacgen's anaerobic bacterial transformation methods typically include electroporation, chemical transformation, or heat shock transformation, depending on the host bacterium's type and characteristics. Post-transformation, screening involves colony PCR, plasmid extraction, and enzyme digestion to identify positive clones containing the target gene.
4. Expression Condition Optimization:
   Optimizing expression conditions is a critical step for anaerobic bacterial system protein expression. It involves adjusting factors such as culture conditions, media components, temperature, pH, and dissolved oxygen levels. The goal of optimization is to find conditions most favorable for efficient target protein expression, achieving high expression levels and optimal protein quality.
5. Protein Purification:
   Drawing on extensive experience in protein purification, we select methods based on the characteristics and requirements of the target protein. Profacgen's purification techniques include affinity chromatography, ion exchange chromatography, gel filtration, ultrafiltration, and dialysis. Throughout the purification process, we closely monitor protein quality and purity, conducting necessary analyses and identifications.

Our Advantages

• Efficient expression optimization
• High cost-effectiveness
• Environmentally friendly
• Wide adaptability
• One-stop service

Case Study

Plasmid construction QC result

Restriction digestion verification and PCR identification of positive strains

Protein expression identification

FAQs