Bio-Layer Interferometry (BLI)

Profacgen provides a comprehensive panel of services for the study of protein-protein interactions, of which the Bio-layer Interferometry (BLI) analysis is commonly used by our customers for the quantitative and qualitative characterization of biomolecule interactions and other applications.

The BLI biosensor platform, developed by ForteBio, is a label-free detection system for molecular interaction analysis, based on the principles of optical interferometry. In this system, the interaction occurs at the very tip of a glass fiber-based BLI biosensor. White light travels down the glass fiber and is reflected back up from two interfaces at the tip: internal interface and external interface. When molecules bind to the surface of the biosensor, the thickness variation of the external layer of immobilized protein causes a shift of the interference pattern. The pattern can be plotted as a nanometer shift of relative intensity vs. wavelength that can be recorded in real-time with high accuracy.

Schematic representation of the principle of the BLI system ^[1]

Profacgen provides label-free and real-time BLI analysis of a wide range of interacting molecules. Our analysis system supports high throughput protein characterization and screening. It has been proven to be reliable by clients from pharmaceutical and diagnostic industry as well as from academia.

Applications:

•   Protein quantitation in the analyte
•   Binding specificity and cooperativity determination
•   Affinity and Kinetics analysis (measurement of reaction rate constants (k_obs, k_a, k_d) and binding affinity constant (K_D))

Key features of the BLI system:

•   High flexibility allowing quantitation ranging from ng/ml to mg/ml
•   Label-free and real-time analysis
•   Rapid test that enables high process efficiency and productivity
•   Relatively low cost
•   Crude sample compatibility (Insensitivity to pH of the matrix or changes in refractive index)

Please feel free to contact us for more details of our BLI service. Our team can help with customized project plan according to your specific requirements.

References:

[1] Roh C, Kim S E, Jo S K. Label free inhibitor screening of hepatitis C virus (HCV) NS5B viral protein using RNA oligonucleotide. Sensors (Basel), 2011, 11(7): 6685-96.