In Vitro Glyco Engineering (IVGE)

Protein is an important source of drug development and industrial enzyme preparation. It is important to reduce side effects and improve the physical and biological properties of engineered proteins. Glycosylation is a common modification of proteins. The process of changing the glycosylation to alter the properties of proteins is known as glycoengineering. The importance of glycoengineering in overcoming the limitations of proteins has been established by research in many disciplines.

Figure 1. Protein glycosylation

As the field of high-throughput screening technology and biochemistry continues to update, in vitro glyco engineering is becoming increasingly important due to its rapid development speed and low time and other costs. Profacgen uses IVGE techniques to optimize some necessary glycotransferases (such as sialic acid transferase and galactosyltransferase) for the drug development phase by modifying glycosidic bonds or enriching certain sugar types (green in Figure 2) using discrete enzyme reactions with clear kinetics and predictable results. IVGE can modify glycosylation in a controlled manner after proteins are produced and purified, and thus can isolate glycosylation management from cell culture manipulations.

Figure 2. N-connectans on glycoproteins produced in different expression systems

Services

We are now integrating IVGE into the downstream purification phase of bioengineering and can now specifically and effectively change the glycation type, and thus the bioactivity, all of the manufacturing complicating GMP requirements. The feasibility of adopting the IVGE process at a manufacturing scale needs to be tested and optimized for each application. The services we provide include but are not limited to:

• Introduction of target glycans at specific sites
• Incorporate additional glycosylation sites in the protein backbone
• Deglycosylation of glycoproteins produced in host cells

Profacgen has accumulated lots of experience in protein engineering. Our professional technical team can provide customers with high-quality in vitro glycol engineering and many related featured services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.

Reference

1. Bo, M.; et al. Protein Glycoengineering: An Approach for Improving Protein Properties. Frontiers in Chemistry. 2020.