Mammalian Cell Expression

Mammalian cell expression systems are the best choice for the production of eukaryotic proteins, especially when proper folding and post-translational modifications (glycosylation, phosphorylation, acetylation, etc.) are required. They produce eukaryotic recombinant proteins in the most natural state, with native tertiary structure, physicochemical properties and bioactivities. This system has been successfully applied to express complicated, modified, functional proteins/antibodies; secreted or membrane proteins such as recombinant glycoproteins (vaccines and enzymes), hormones, monoclonal antibodies, and cytokines.

The most widely used mammalian cell lines are Human Embryonic Kidney 293 (HEK293) and Chinese Hamster Ovary (CHO) cells. HEK293 cells have been extensively used for the transient expression of recombinant proteins, and their ease of culture and efficient transfection rates make them particularly suitable for customized protein production for research purposes.

CHO cells have been successfully used to manufacture FDA-approved therapeutic proteins and antibodies, and prominent in stable expression. Stably transfected CHO cell lines are the most suitable mammalian hosts for high-yielding recombinant protein production. They are adaptable to diverse culture conditions, and protein purification from CHO culture is also relatively easy because of their secretory feature.

Profacgen provides you with an ultimate solution for mammalian protein production. Our service can be tailored to your specialized requirements. Our expertise guarantees high protein quality, short turnaround time, and the best price in the market!

Services Details

• Gene Synthesis
  Gene synthesis
  Codon optimization in mammalian expression systems
  Subcloning
• Pilot Study
  Transfection
  Small-scale protein expression
  Optimization of expression conditions
• Expression Amplification and Purification
  Large-scale protein expression
  Purification: affinity chromatography, gel filtration, ion-exchange
  QC analysis: SDS-PAGE, Western blot, mass spectrometry
• Stable Transfection
  Stable transfected cell line construction

Additional Services

• Protein Labeling
  Profacgen offers a range of protein labeling technologies, including fluorescent probes (FITC), isotope labeling, biotinylation, and enzyme conjugates.
• Large-scale Production
  Advanced bioreactors with precise control of process conditions such as incubation temperature, pH, dissolved oxygen and pressure.

See more details on the principle and protocol of mammalian cell expression system on our website.

Profacgen has accumulated lots of experience in protein expression. Our professional technical team can provide customers with high-quality mammalian cell expression system and many related featured services you may be interested in. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.