Lettuce Transient Expression System
Commonly-used recombinant protein expression systems include bacterial, yeast, insect, and mammalian cell culture systems. With the rapid development of plant transgenic technology, the plant expression system has been widely applied in both agricultural and plant biological studies. It has been reported that many biological products (such as bioactive peptides, therapeutic proteins) were generated from plant systems such as tobacco, tomato, rice and lettuce. Transient expression of target proteins in the plant systems is realized by agroinfiltration of plant tissues. Following successful agrobacterial transformation, target recombinant proteins, such as antibodies, vaccines, and enzymes can be expressed in high amount within the plant system. Comparing to the traditional bacterial, yeast, or mammalian expression systems, the plant expression system has the advantages of higher protein yield, lower cost, shorter turn-over time and increased safety. Among all plant species, the lettuce transient expression system is considered as the most promising system for the expression of recombinant proteins.
Lettuce is a self-fertile, diploid plant species with robust growth capability, which are easy to cultivate and has therefore become a popular model for the application of plant heterologous expression system. So far, a list of recombinant proteins have been successfully expressed in lettuce, such as the human intestinal trefoil factor, the human thioredoxin-1 protein, insulin, interferons, thymosin, as well as a couple of antibodies.
Recombinant gene transformation can be performed with different tissues of the lettuce, including leaf and stem. For the transformation with leaf tissues, after agroinfiltration, the target protein can be expressed within 2–3 days. The transformed leaf is then harvested, and subjected to protein extraction and purification. Comparing to the conventional stable expression, the transient express approach yields higher protein expression levels. Aside from the higher expression efficiency, our in-house plant factories ensure that no transgenic product is exposed to the environment, which is more safe and environmental-friendly.
Features:
Low production cost
Shorter set-up time
Shorter turnaround time
Large-scale production
Convenient storage
Capability of complex protein expression
Profacgen provides you with an ultimate solution of lettuce expression system. Our service can be tailored according to your specialized requirements. Our expertise guarantees high protein quality, short turnaround time, and the best price in the market!
References:
[1] Joh LD, Wroblewski T, et al. High-Level Transient Expression of Recombinant Protein in Lettuce. Biotechnol Bioeng. 2005; 91: 861-871.
[2] Chen Q, Dent M, et al. Transient Protein Expression by Agroinfiltration in Lettuce. Methods Mol Biol. 2016; 1385: 55-67.
[3] Dong H, Zhao Y, et al. Recombinant proteins expressed in lettuce. Indian Journal of Biotechnology. 2014; 13: 427-436.