Single Molecular Array Testing

Protein is the ultimate executor of life functions. It is also an essential and widely used detection object in biomedical-related fields such as clinical diagnosis, disease typing, and drug screening. Traditional ELISA technology has always been the primary method in protein detection. However, although ELISA can be applied to almost all protein detection, its sensitivity is poor, generally only reaching the level of pg/mL. This makes the detection of low-abundance proteins often tricky. In drug research, it is found that the accurate detection of these ultra-low abundance proteins is of great significance for predicting the occurrence of diseases. Therefore, a protein detection technology with high sensitivity is urgently needed.

Single Molecular Array (SiMoA) technology is a newly developed technology that can be thousands of times more sensitive than ELISA. It brings protein detection technology directly into the era of single-molecule and digital detection and detects ultra-low abundance proteins with the accuracy of fg. The biological principle of Simoa detection is still the classical immunoreaction-double-antibody sandwich method, but the Simoa technology coats about 250,000 capture antibodies on small 2.7 μm magnetic beads. During detection, biotin-labeled detection antibody and avidin-coupled enzyme and substrate were added. A single magnetic bead was blocked in 238,000 4.5 μm reaction wells (Wells) through a layer of oil for reaction.

Figure 1 Single Molecule Arrays (Simoa™) technology.^[1]

Profacgen has rich experience in protein research. With our professional scientists and the most advanced equipment, we can provide customers with the detection of various protein modifications with the fastest delivery time and the most accurate delivery results.

SiMoA's technical advantage:

• High Sensitivity and High Accuracy

SiMoA is, on average, more than 1000-fold more sensitive than existing conventional immunoassays, can detect analytes at deficient concentrations, and provides a powerful tool for detecting and validating new biomarkers. Detects dilutions of small sample volumes, saving sample volume while reducing matrix effects. Profacgen offers highly sensitive and highly accurate SiMoA assays to provide accurate results for your low and ultra-low abundance samples.

• Fully Automated

Current immunoassays are labor-intensive, time-intensive, or expensive automated systems. The SiMoA assay can automatically complete all the detection work of ELISA, providing an efficient and convenient detection process and reducing manual operation error.

• High-throughput detection

SiMoA can detect multiple target molecules simultaneously in the same sample. This saves costs and reduces the amount of samples. It has unique advantages for high-throughput detection. 288 samples can be detected at the same time each time, and 500 samples can be detected in one day.

• Variety of sample types

As a technology that can perform ultra-low abundance detection, SiMoA can detect various sample types, including but not limited to serum, plasma, urine, saliva, cell lysates, etc.

With advanced technology and rich experience, Profacgen provides SiMoA analysis tests for a variety of target proteins and also provides other detection methods, such as ELISA, for customers to choose from, which can be guaranteed for your projects and research.

Please get in touch with us for specific project implementation content and time. If you want to know more service information, please feel free to contact us; Profacgen will be your trusted partner.

Reference

1. Fischer SK, Joyce A, Spengler M, Yang TY, Zhuang Y, Fjording MS, Mikulskis A. Emerging technologies to increase ligand binding assay sensitivity. AAPS J. 2015 Jan;17(1):93-101. DOI: 10.1208/s12248-014-9682-8.