Single-cell Enzyme Assay

Cell is the basic unit of the structure and activity of life body, so it is necessary to take cell as the research basis to understand some rules of life activity in life body. For the study of individual cells, more accurate and comprehensive cell information can be grasped, which is of great significance for the study of other disciplines and the early prevention and diagnosis of diseases. Single cell enzyme activity analysis is a real-time, in situ, minimally invasive signal detection and analysis technology to determine a variety of signaling molecules, biomarkers and enzyme activities at the subcellular and molecular levels of single cells. At present in the single-cell level of enzyme activity analysis mainly based on the following two aspects: image-based analyses and flow-based systems.

 Image-based single-cell enzyme analysis techniques are performed using microscopes in combination with fluorescence substrates, or FRET substrates, which are based on the principle of fluorescence Resonance Energy transfer (FRET). Early analyses used an oil layer to confine single cell lysates to individual nanoliter-volume droplets. We currently adopt the latest microfluidic device, while early analyses used an oil layer to confine single cell lysates to individual Nanoliter-volume droplets.

The main steps of the microfluidic analysis technique we provide are as follows:

• Using the microfluidic device makes the cell solution phase and peptide solution via two channels together after further micro-droplet formation, micro-droplet encapsulation of single-celled and polypeptide, micro droplet flow under the drive of continuous phase, in which peptide solution containing molecules can be induced to enhance enzyme secretion peptide sequence including specific enzyme fragment, and fragments of head and tail connection with fluorescence, respectively groups and quenched groups.
• Collect microdroplet and detect the signal with fluorescence microscope.
• Determine enzyme activity according to fluorescence signal.

Flow-based systems represents the gold standard for high-throughput single-cell measurements. Flow cytometric analyses often use fluorescent antibodies to measure enzyme (or phosphoenzyme) concentration as a proxy for enzyme activity.

Why Choose Profacgen?

• Has short and quality testing period, low cost, complete experimental scheme, and scientific, fair and accurate testing report issued.

• With the international advanced testing equipment, has a special laboratory for scientific research.
• All experiments sign confidentiality agreements and pay attention to the protection of customer privacy.
• Ensure 24/7 online service.

Workflow of Single-cell enzyme assays

Our laboratory keeps a close eye on the development of science and technology and uses the latest test equipment and methods. If you are interested in our single-cell enzyme assay services, please feel free to contact us. We look forward to cooperating with you.