MeRIP-Seq Service
MeRIP-Seq (methylated RNA immunoprecipitation sequencing) refers to the combination of RNA-protein co-immunoprecipitation and high-throughput sequencing technology, which can realize RNA methylation analysis at the whole transcriptome level. RNA methylation refers to the methylation modification phenomenon that occurs at different positions on the RNA molecule. Standard RNA post-transcriptional modification methods include 6-methyladenosine, m6A, and C5-methylcytidine, m5C.
Since methylation modification accounts for more than 60% of all RNA modifications, N6-methyladenosine (m6A), the most common post-transcriptional modification, mediates more than 80% of RNA methylation. m6A modification plays an important role in regulating gene expression, splicing, RNA editing, RNA stability, controlling mRNA lifespan and degradation, mediating circular RNA translation, etc. Therefore, meRIP-Seq helps to solve biological problems such as cell differentiation, biological development, and disease occurrence and development. The analysis of RNA methylation patterns based on MeRIP-Seq has accelerated the research on biological functions and mechanisms of cancer occurrence and metastasis, embryonic development, lipid metabolism, circular RNA translation, and DNA damage repair.
Figure 1. Model of MeRIP-seq.[1]
MeRIP-Seq sample requirements:
- Tissue: >50mg; samples with low RNA content require more tissue.
- Cells: 5x106~107; samples with low RNA content need more cells.
- Total RNA: >50ug.
meRIP-Deq analysis project:
| Fundamental Analysis | Advanced Analysis |
| Remove adapters and quality control QC. | Association analysis (with RNA-seq or multi-omics association analysis) |
| Reference Genome Alignment (Mapping) | Association Analysis Heatmap (Heatmap) |
| Enriched region identification (Peakcalling) | IGV peak diagram |
| Annotation of enriched regions (PeakingAnno) | |
| Motif Analysis | |
| Peak gene GO and KEGG analysis. | |
| Differential Peak analysis (differential RNA modification mRNA, lncRNA) | |
| Differential Peak gene GO and KEGG analysis. |
Our Advantages:
- One-stop and customized service.
- Rich project experience.
- Perfect platform.
- Staff scientists for each project.
With advanced technology and rich experience, Profacgen provides MeRIP-Seq service for service samples. According to the information of customer samples, customize the program for customers to provide better analysis results.
Please get in touch with us for specific project implementation content and time. If you want to know more service information, please feel free to contact us; Profacgen will be your trusted partner.Reference
- Cui, Xiaodong & Meng, Jia & Rao, Manjeet & Chen, Yidong & Huang, Yufei. (2015). HEPeak: An HMM-based exome peak-finding package for RNA epigenome sequencing data. BMC Genomics. 16. S2. 10.1186/1471-2164-16-S4-S2.