Protein-protein interactions (PPIs) are established when protein molecules physically contact with each other through hydrogen or hydrophobic bonds, Van der Waals forces, ionic forces, or even covalent bonds. This phenomenon underlies a wide range of essential cellular events such as signal transduction, molecular transport and various metabolism pathways. PPIs are also the basic of many diseases. Therefore, PPIs have been studied extensively in the area of bioscience and medical research.

Protein Interaction Analysis Services

Profacgen has organized a team of scientists with extensive experience in the study of PPIs to offer you one-stop service on your PPI project. We can help with your experiment design, carry out your experiments, and perform data analysis. We have established in our lab a series of assays to help with your research, including high throughput interaction screening assays such as Yeast two-hybrid screening and phage display technology, interaction strength and kinetics assays such as Surface Plasmon Resonance (SPR). Each approach has its own advantages and weaknesses in terms of sensitivity and specificity. While you can choose the techniques your prefer, we can also provide our recommendations.

Please see the list of assays that we provide below:

Yeast Two-Hybrid Screening
Membrane-based Yeast Two-Hybrid Screening
Surface Plasmon Resonance (SPR)
Co-Immunoprecipitation (Co-IP)
Pull-Downs
Bio-layer Interferometry (BLI)
Isothermal Titration Calorimetry
Protein Binding Site Mapping
Biomembrane Force Probe (BFP)
Microscale Thermophoresis (MST)
Tandem Affinity Purification (TAP)-MS
Yeast Display Service
Fluorescence Resonance Energy Transfer (FRET)
Screening Interacting Proteins by Protein Chip
SILAC-IP-MS
Circular Dichroism (CD)
Nuclear Magnetic Resonance (NMR)
Bimolecular Fluorescent Complimentary (BiFC)
Membrane Protein Binding Screening and Profiling
Enzyme Fragment Complementation Assay (EFCA)
Bioluminescence Resonance Energy Transfer (BRET)
Affinity- Purification /mass Spectrometry (AP-MS) Service
Proximity-dependent Biotin Identification (BioID) Service

Please feel free to contact us with your protein-protein interaction projects. Profacgen is here to offer you professional and thoughtful service!