Baculovirus-Mammalian cell Protein Expression System
The baculovirus expression vector system (BEVS) is commonly used to express target genes in insect host systems. Due to yield limitations, BEVS is rarely applied to mammalian host cells. Profacgen inserts mammalian cell-active expression cassettes into baculoviruses to efficiently express genes into many different mammalian cells, greatly increasing protein production through transfection. Our BEVS has the advantages of broad cell tropism, transient viral expression, and ease of generation. It enables rapid, easy and flexible protein expression and can be applied to a variety of mammalian cell lines such as CHO cells and HEK293 cells.
BacMams are genetically optimized baculoviruses in which a mammalian-driven promoter is used to express genes of interest, allowing proper protein expression in mammalian cells following baculovirus transduction. The protein yield of the commonly used lipid-mediated transient transfection system in mammalian cells is generally 0.5-10 mg/L. Thanks to the excellent work of Profacgen scientists, the yield of baculovirus-mammalian cell protein expression has been increased to over 10 mg/L. This system has been successfully applied to the expression of proteins with low endogenous content, high expression difficulty and/or complex structures.
Figure 1. Baculovirus vectors can be used for a variety of applications (Kost, T.A.; et al. 2005)
Our Services
Profacgen provides professional baculovirus-mammalian cell protein expression services. With our experienced and dedicated team of scientists, we provide our clients with high-quality protein expression and stable cell line development services. The bacmam plasmid we developed containing the bicistronic expression cassette enables gene delivery in many different cell types by simply adding a viral inoculum. Through extensive experiments, we also explored many parameters that can improve cell transduction, gene expression, and protein production, such as:
- Dilute viral inoculum with phosphate-buffered saline
- Low temperature incubation
- Add the virus multiple times
- Various additives such as enzyme inhibitors, salts, etc.
Profacgen has accumulated lots of experience in protein expression. Our professional technical team can provide customers with a high-qualitybaculovirus-mammalian cell protein expression system and many related featured services you may be interested in. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.
References
- Kost, T.A.; et al. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature biotechnology. 2005.
- Lackner A.; et al. Stable Protein Expression in Mammalian Cells Using Baculoviruses. Methods in Molecular Biology. 2012.