Insect Cell Expression

In contrast to bacteria and yeast, insect cells are higher eukaryotic systems that closely resemble mammalian cells and are capable of expressing large quantities of recombinant proteins with correct folding and high order posttranslational modifications (PTMs), such as some human proteins, that cannot be produced using protein expression in bacteria.

The baculovirus expression vector system (BEVS) is a powerful tool for expressing target genes in insect cells such as Sf9 and Sf21. Because BEVS can be incorporated into DNA fragments without size restriction, it is ideal for the expression of large-sized proteins. The system is also known for high-yield production because multiple copies of the gene of interest can be incorporated. It is worth noting that insect cells can co-express more than one exogenous gene simultaneously, which enables the expression of multi-protein complexes and is an efficient way to study protein interaction networks. In addition to this, insect cells are also a recognized choice for membrane protein production. The function of membrane proteins is often associated with disease states, and membrane proteins can be expressed and purified by insect cell expression systems while preserving their structural and functional integrity.

Spodoptera frugiperda is part of the order of Lepidoptera.
Spodoptera frugiperda cells (Sf9 and SF21 cell lines) are commonly used in biochemical research for recombinant protein expression using insect-specific viruses called baculoviruses.

Drosophila melanogaster is a species of fly in the family Drosophilidae.
D.melanogaster is one of the most widely used and genetically best-known of all eukaryotic organisms.

Profacgen provides you with an ultimate solution for insect cell expression. Our service can be tailored to your specialized requirements. Our expertise guarantees high protein quality, short turnaround time, and the best price in the market!

Service Details

• Expression vector construction
  Codon optimization
  Gene synthesis
  Subcloning
• Virus generation
  Bacmid DNA generation
  Transfection of insect cells with the recombinant Bacmid
  Generation of P1 stock (Low titer) and P2 stock (High titer)
  Determination of virus titer
• Expression evaluation
  Infection of insect cells with P2 stock
  Expression optimization
  SDS-PAGE gel and Western blot
• Scale up and purification
  Expression and purification with optimized conditions
  QC

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