Incorporation of Bioorthogonal Handles
Proteins are the most prevalent biomolecules in the cell and participate in virtually all cellular processes. Thus, selective labeling of proteins is of significant importance for understanding structural and functional properties of proteins. Green fluorescent protein (GFP) and its variants are believed to be the most powerful tool for protein visualization. However, due to their relatively large size and other drawbacks, many small molecular-based alternative methods have been developed over the years, of which bioorthogonal reactions are the most reliable and invaluable tools for protein labeling.1 Typically a bioorthogonal handle is first incorporated into a protein to enable site-specific modification. Then, various labels can be installed onto the protein via bioorthogonal reactions.
As an industry-leading organization, Profacgen has established a reliable and highly efficient platform for the incorporation of varying bioorthogonal handles into proteins of interest, providing the least invasive approach for protein labeling and modification with remarkable ease.
Hundreds of customers have endorsed the advantages of choosing Profacgen:
- Highly customized service to meet specific needs;
- Large selection of bioorthogonal handles and incorporation protocols;
- Interdisciplinary research team with well-experienced technicians;
- Full support from ideas, through process trouble-shooting, to delivery.
For more information regarding Profacgen's incorporation of bioorthogonal handles service, please contact us. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
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Referecnes
Chen, X.; et al. Selective chemical labeling of proteins. Organic & Biomolecular Chemistry 2016, 14 (24): 5417-5439.