Multi-protein Expression Service in Insect Cells

Multi-protein complexes play important roles in a wide range of cellular processes, including transcription, translation, RNA splicing, signaling cascades, protein trafficking and degradation. With the deepening of research, scientists realize that the function of a protein depends not only on its own physicochemical properties, but also on its interactions with its partners, and the characteristics of them, so more and more studies begin to focus on protein complex's structure and function. Protein complexes have shown important roles in the biotechnology industry, metabolic engineering and even in the biopharmaceutical therapy, and the strategies of reconstructing multi-proteins through protein co-expression has become widely used methods.

The Baculovirus Expression Vector System (BEVS) is a powerful platform for protein expression in insect cells. This system is capable of post-translational modifications similar to mammalian cells and is inherently safe during high-level expression. Therefore, the use of BEVS to conduct protein co-expression studies in insect cells is favored by more and more researchers.

Figure 1. Baculovirus life cycle and gene expression

Different strategies of co-expression services provided by Profacgen:

• Single polycistronic baculovirus (co-expression)

Proteins are expressed from single baculoviruses carrying multiple foreign genes (polycistronic)

• Multiple monocistronic baculoviruses (co-infection)

Proteins are expressed from multiple baculoviruses each carrying a single foreign gene (monocistronic)

• A combination of the two

BEVS co-expression and co-infection have been used to:

• Compound structure-function research
• Functional analysis of protein domains
• Complement or augment the ability of cells to produce foreign proteins or protein systems
• Production of protein complexes (including VLP production)

Profacgen has accumulated extensive experience in the field of baculovirus-insect cell expression systems and provides high-quality protein co-expression services to help scientists conduct protein co-expression network analysis and interaction studies. Our highly competitive prices and unprecedented expertise have earned us the trust of a large number of researchers. Request more information to find out how Profacgen could be assistance.

Reference

1. George, S. Use and Control of Co-expression in the Baculovirus-Insect Cell System for the Production of Multiple Proteins and Complex Biologics.