DAP-seq Service (DNA affinity purification sequencing)

DNA affinity purification sequencing (DAP-seq) was used to construct transcription factor protein in vitro, bind to the target genomic DNA fragment, and enrich the genomic DNA fragment bound to the target protein. By combining with high-throughput sequencing technology, the DNA products after DAP were sequenced and analyzed to find the DNA binding sites of the target protein from the whole genome, and high-throughput data results were obtained by efficient sequencing methods. DAP can effectively solve the limitation of ChIP technology which lacks the specific antibody of target protein, and has a wider range of application.

Figure 1. DAP-seq protocol overview (Bartlett, A.; et al. 2017)

In the past epigenetic research, the discovery of regulatory sites of transcription factors has always been a difficult problem. ChIP, as a classical technology, has many disadvantages, such as low throughput and nonspecific antibody, which are difficult to solve. In order to map the binding sites of hundreds quickly and completely or even thousands of known transcription factors, a more rapid method is needed to map these loci to obtain the cis and trans group map. In this system, a labeled transcription factor can be added to the DNA library, which can bind to DNA randomly. Then all paired DNA proteins can be isolated. DAP-seq, as an in vitro experimental technique with the same function of ChIP, is a sharp edge in the study of cis and trans group of model species.

Services

As an expert in protein-nucleic acid interaction research, the DAP-seq technology platform established by Profacgen enables high-throughput creation of genome-wide binding site maps, and efficiently analyze the effects of modifications in genomic DNA libraries on transcription factor binding.

Our service process includes:

• Transcription factor expression vector construction
• Cell-free expression of transcription factor proteins
• DAP-seq library construction
• DNA affinity purification
• Illumina Next Generation Sequencing
• Data Processing and Analysis

You only need to provide the transcription factor sequence and genome sample of the material to be tested, and Profacgen can complete the DAP-seq service for you. We deliver high-throughput sequencing raw data, bioinformatics analysis reports, and transcription factor binding motif sequences.

Profacgen has accumulated lots of experience in nucleic acid-protein interaction research. Our professional technical team can provide customers with efficient DAP-seq and many related featured services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.

References

1. Bartlett, A.; et al. Mapping genome-wide transcription-factor binding sites using DAP-seq. Nat Protoc. 2017.
2. Li, M.; Huang, S.C.; DNA Affinity Purification Sequencing (DAP-Seq) for Mapping Genome-Wide Transcription Factor Binding Sites in Plants. Accelerated Breeding of Cereal Crops. 2022.