Transmembrane proteins are a class of proteins embedded in the phospholipid bilayer of cell membrane to realize the intracellular and extracellular spanning. The interaction of transmembrane region is an important channel to connect the extracellular environment and intracellular environment, so as to perform various activation and response responses, realize signal transduction, and regulate the morphological and functional changes inside and outside the cell. Many human diseases may be related to the abnormal function of transmembrane proteins. The versatility of transmembrane proteins makes them ideal drug targets.
Multiple transmembrane proteins have complex structure and multiple hydrophobic transmembrane regions, which are often expressed at a very low level in host cells and difficult to prepare. No matter what kind of antibody separation technology is used, the preparation of transmembrane protein antigen with natural conformation and complete activity is very important for the successful separation of transmembrane protein antibody with therapeutic function, which has become an urgent problem.
VLP is the abbreviation of virus like particle. VLP comes from the outer capsid protein of the virus, which is composed of one or more capsid protein components automatically assembled into nanoparticles. VLP particles do not contain the infectious genome of virus, so it is very safe in the process of production and operation. With the development of technology, based on the effect that VLP can effectively activate humoral and cellular immune response, the role of VLP display antigen in the discovery of high difficulty target antibody is obvious, with great advantages.
Enveloped VLPs directly aggregate membrane proteins with complete conformation on the cell surface, making these complex membrane proteins into soluble and high concentration proteins for antibody immunization and screening. Profacgen has specially built a platform of enveloped VLPs technology based on HEK293 expression system, and the prepared enveloped VLPs display correctly folded multiple transmembrane proteins on their inherent cell membrane, Thus, we can induce and screen the functional antibodies that recognize the natural conformation of the target.
In order to contribute to the global innovation of antibody drug research and development, Profacgen provides a series of Enveloped VLPs technology for membrane protein production based on its exclusive new functional recombinant protein and antibody R&D and production platform. If you are interested in our Enveloped VLPs technology, Profacgen looks forward to cooperating with you.
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