A New Platform for Obtain Plant Plasma Membrane Protein
Plasma membrane, once called the cell membrane, refers to an extremely thin membrane that surrounds the cell surface and is mainly composed of membrane lipids and membrane proteins, usually includes the cell's inner membrane system (organelle membrane and nuclear membrane) and cell membrane. The basic function of the plasma membrane is to maintain the relative stability of the intracellular microenvironment, and to participate in material exchange, energy, and information transfer with the external environment. In addition, it plays an important role in the survival, growth, division, and differentiation of cells.
The cytoplasm of plant cells is also surrounded by a plasma membrane, and this plasma membrane has the unique function of selective permeability. All material exchange between plant cells and the external environment must pass through the plasma membrane. The effects of various adverse environmental factors on cells often first act on this biofilm composed of lipids and proteins. However, the plant membrane system including cell membrane, nuclear membrane and endoplasmic reticulum, Golgi apparatus, mitochondria, chloroplast, and other organelles surrounded by membranes exists in insoluble form during the separation and extraction process. The study of plant plasma membrane has been severely limited and there has been no suitable research platform in vitro. Therefore, the research on plant plasma membrane has been stagnant.
Recently, researchers have developed a new platform that can be used to study the plasma membrane of plant cells. This platform can retain a variety of components on the plasma membrane, including lipids, proteins, and other components, and allows the use of various molecular biology tools in the researches. The platform relies on the supported lipid bilayers platform and optimizes the platform by using the transport of cell plasma membrane vesicles, using its own unique materials for in vitro models, to preserve the function and native orientation of membrane proteins. With its unique advantages, this platform has shown great potential in the study of cytoplasmic membranes, which is beneficial to the establishment and measurement of in vitro models in various biological research fields.
The Advantages of this platform include:
- No need to purified proteins
- Preserve the function and native orientation of membrane proteins
- Compatible with molecular biology tools
- Preserve the constituents of membrane proteins
Potential Applications
- Research on plant membrane proteins
- Research on host cell-pathogen interactions
- Tissue engineering
- Studies of plant molecular biology
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Reference
- Castellana ET, Cremer PS. Solid supported lipid bilayers: From biophysical studies to sensor design. Surf Sci Rep. 2006;61(10):429-444. doi:10.1016/j.surfrep.2006.06.001.