Product description
Xanthine oxidase (XOD, EC 1.17.3.2) is a type of aerobic dehydrogenase, one of the main sources of reactive oxygen species, and a key enzyme in nucleotide metabolism. XOD is primarily found in tissues such as the liver of mammals. When liver function is impaired, XOD is released into the serum in large amounts, which has specific significance for the diagnosis of liver damage. XOD catalyzes the oxidation of xanthine to uric acid and superoxide anion radicals, which then react with a colorimeter to form colored substances. The XOD enzyme activity can be calculated by measuring the amount of colored substance generated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature centrifuge, pipette, mortar, ice, and distilled water