Product description
Trypsin (EC 3.4.4.4) is a type of protease that can catalyze the hydrolysis of amide bonds. It is an important digestive enzyme. Trypsin catalyzes the hydrolysis of N-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA) to produce p-nitroaniline, which has a maximum absorption peak at 405nm. The activity of trypsin can be determined by measuring the rate of increase in absorbance.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, pipette, balance, mortar and pestle, ice, and distilled water.