Product description
Trehalose is a non-reducing disaccharide widely found in animals, plants, microorganisms, and cultured cells. It has the role of protecting biomolecules such as nucleic acids and proteins in adverse environments such as dryness, drought, freezing, and high osmotic pressure, and is widely used in the preservation of pharmaceuticals, health products, enzymes, food, and other products. This kit uses the anthrone-sulfuric acid method to determine the content of trehalose. Under the action of concentrated sulfuric acid at higher temperatures, sugars can be dehydrated to form furfural or hydroxymethyl furfural, which then condenses with anthrone to form derivatives of furfural, a blue-green substance. This substance has maximum absorption at 620nm, and the depth of its color is directly proportional to the sugar content.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), water bath, pipette, mortar and pestle, concentrated sulfuric acid, and distilled water.