Product description
Trehalose-6-phosphate synthase (TPS, EC 2.4.1.15) is one of the key enzymes in trehalose synthesis, catalyzing the conversion of UDP-glucose and glucose-6-phosphate to trehalose-6-phosphate and UDP. UDP and phosphoenolpyruvate are sequentially acted upon by pyruvate kinase and lactate dehydrogenase, oxidizing NADH to NAD+. By detecting the decrease in NADH at 340nm, the enzyme activity of TPS can be calculated.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvette (light path 1cm), desktop centrifuge, water bath/thermostat/metal bath, pipette, mortar and pestle, ice.