Product description
Trehalose-6-phosphate phosphatase (TPP, EC3.1.3.12) is one of the key enzymes in trehalose synthesis, catalyzing the conversion of trehalose-6-phosphate to trehalose. This kit utilizes the enzyme to catalyze the substrate trehalose-6-phosphate to produce trehalose, which is then broken down into glucose by trehalase and reacts with a specific color reagent under the action of glucose oxidase to form a colored substance. By detecting the value of the colored substance at 520nm, the activity of TPP can be determined.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, water bath/thermostat/metal bath, pipette, mortar and pestle, ice.