Product description
Trehalose-6-phosphate phosphatase (TPP, EC3.1.3.12) is one of the key enzymes in trehalose synthesis, catalyzing the conversion of trehalose-6-phosphate to trehalose. This kit utilizes the enzyme to catalyze the substrate trehalose-6-phosphate to produce trehalose, which is then broken down into glucose by trehalase and reacts with a specific color reagent under the action of glucose oxidase to form a colored substance. By detecting the value of the colored substance at 520nm, the activity of TPP can be determined.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), desktop centrifuge, water bath/thermostat/metal bath, pipette, mortar and pestle, ice.