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Total Ascorbic Acid (TAA) Assay (based on 2,4-Dinitrophenylhydrazin) (ASGQT-K003M)

PRODUCT INFORMATION

Cat.No.
ASGQT-K003M
Method
Microplate-based method
Product size
96T
Note
Provide microplate
Product description
Total Ascorbic Acid (TAA) includes both reduced and dehydroascorbic acid. Reduced ascorbic acid is easily oxidized to dehydroascorbic acid, which reacts with 2,4-dinitrophenylhydrazine to form a red complex soluble in sulfuric acid. This red substance has a maximum absorption peak at 520nm, from which the total ascorbic acid (TAA) content is calculated.
Additional Materials and Equipments Required
Plate reader, 96-well plate, concentrated sulfuric acid, mortar and pestle, ice, low-temperature centrifuge, pipette, and distilled water.
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