Total Ascorbic Acid (TAA) Assay (based on 2,4-Dinitrophenylhydrazin) (ASGQT-K003M)

Cat.No.

ASGQT-K003M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Total Ascorbic Acid (TAA) includes both reduced and dehydroascorbic acid. Reduced ascorbic acid is easily oxidized to dehydroascorbic acid, which reacts with 2,4-dinitrophenylhydrazine to form a red complex soluble in sulfuric acid. This red substance has a maximum absorption peak at 520nm, from which the total ascorbic acid (TAA) content is calculated.

Additional Materials and Equipments Required

Plate reader, 96-well plate, concentrated sulfuric acid, mortar and pestle, ice, low-temperature centrifuge, pipette, and distilled water.