Product description
Alkaloids are nitrogen-containing organic compounds found in organisms, mostly in plants, with over three thousand types isolated to date, including nearly a hundred with strong physiological activity widely used in clinical medicine. Alkaloids react with bromophenol green to form a yellow substance with a characteristic absorption peak at 415nm. By detecting the increase at 415nm, the total alkaloid content in the sample can be determined.
Additional Materials and Equipments Required
Spectrophotometer, 1mL quartz cuvette (light path 1cm), constant temperature water bath, desktop centrifuge, pipette, dichloromethane, methanol