Product description
Succinate dehydrogenase (SDH, EC 1.3.5.1) is widely present in animals, plants, microorganisms, and cultured cells. In eukaryotes, it is bound to the inner mitochondrial membrane, and in prokaryotes, it is integrated into the cell membrane. It is one of the hubs connecting respiratory electron transport and oxidative phosphorylation. SDH catalyzes the dehydrogenation of succinate to fumarate, and the hydrogen removed is transferred through phenazine methosulfate (PMS) to reduce 2,6-dichlorophenolindophenol (DCPIP), which has a characteristic absorption peak at 600nm. The enzyme activity of SDH can be determined by the change in absorbance at 600nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, low-temperature centrifuge, mortar and pestle.