Product description
Sorbitol, as a transport sugar, is converted into other sugar substances when unloaded into fruits. Sorbitol oxidase (sorbitol oxidase, SOX) is one of the key enzymes in the transformation and utilization process of sorbitol and is closely related to fruit quality and the accumulation of sugar substances in fruits. Sorbitol oxidase (SOX) catalyzes the conversion of sorbitol into glucose, which further reacts with 3,5-dinitrosalicylic acid to form a brown-red amine compound with characteristic light absorption at 540nm. Within a certain range, the rate of increase in light absorption at 540nm is proportional to the activity of sorbitol oxidase (SOX).
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), low-temperature centrifuge, water bath, pipette, mortar and pestle, ice, and distilled water.