Product description
Nucleic acids are one of the components of soil organic phosphorus. Under the action of nucleases, nucleic acids are broken down step by step to release inorganic phosphorus, which is one of the phosphorus sources for plants. The activity of soil nucleases is measured by quantifying the activity of soil nucleotidases. Soil nucleases, i.e., soil nucleotidases, hydrolyze substrate ribonucleotides to produce inorganic phosphorus and other substances. Inorganic phosphorus reacts with ammonium molybdate to form a blue complex in an acidic environment, and the activity of soil nucleases can be calculated by detecting the generation rate of the colored substance at 700nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, low-temperature centrifuge, constant temperature water bath, pipettes.