Product description
Soil hydroxylamine reductase can reduce hydroxylamine, an intermediate product formed during nitrogen metabolism in soil, to ammonia. The reducing compounds in soil can act as donors of hydrogen, and their strength affects the volatilization loss of nitrogen as ammonia during soil nitrogen metabolism, indirectly affecting the efficiency of nitrogen fertilizer utilization. Fe3+ in ammonium sulfate can oxidize hydroxylamine to nitrogen gas and is itself reduced to Fe2+. Fe2+ forms an orange-red complex with o-phenanthroline under weak acid conditions, which has an absorption peak at 510nm. The action of hydroxylamine reductase on hydroxylamine reduces the formation of the complex, and the decrease in absorbance at 510nm can reflect the activity of hydroxylamine reductase.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipettes, balance, room temperature centrifuge, shaker, nitrogen blower.