Product description
Glutamine transaminase (GLS, EC 3.5.1.2) is a type of amidase that catalyzes the hydrolysis of glutamine into glutamate and ammonia, playing a significant regulatory role in nitrogen metabolism. In soil, S-GLS catalyzes the hydrolysis of glutamine into L-glutamate and ammonia. Ammonia, in a strong alkaline environment, reacts with hypochlorite and phenol to form the water-soluble dye indigo blue, which has a stable solution color. It has a characteristic absorption peak at 630nm, and by detecting the rate of increase of ammonia, the enzyme activity can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, pipette, water bath or temperature-controlled incubator, toluene, and distilled water.