Product description
Soil β-glucosidase (β-GC, EC 3.2.1.21) can catalyze the hydrolysis of glycosidic bonds between aryl or alkyl groups and sugar atoms to generate glucose. It is an important component of the cellulase system and has an important physiological function in the sugar metabolism of soil microorganisms. Soil β-glucosidase can catalyze p-nitrophenyl-β-D-glucopyranoside to generate the yellow substance p-nitrophenol (PNP), which has characteristic light absorption at 405nm, thereby obtaining the activity of soil β-glucosidase.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, temperature-controlled shaking incubator/water bath, pipettes, balance.