Product description
Soil phosphatase plays an important role in the availability of soil phosphorus and is an indicator for evaluating the direction and intensity of soil phosphorus biotransformation. It also has a certain relationship with soil carbon, nitrogen content, available phosphorus content, and pH. This kit provides a simple, sensitive, and rapid detection method. In an acidic environment, soil acid phosphatase (S-ACP) catalyzes the generation of yellow p-nitrophenol (PNP) from p-nitrophenyl phosphate (PNPP), which has a maximum absorption peak at 405nm. By detecting the increase rate of PNP at 405nm, the activity of S-ACP enzyme can be obtained.
Additional Materials and Equipments Required
Visible spectrophotometer (wavelength setting: 405nm), 1mL glass cuvettes (optical path 1cm), desktop centrifuge, constant temperature incubator, analytical balance, pipettes, distilled water, toluene.