Recombinant Rat Cst3 Protein(His tag) (HE0006CS)

Recombinant Rat Cst3 Protein (NP_036969) (21-140aa) with C-terminus His tag was expressed in Insect cells.

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PRODUCT INFORMATION

Cat.No.

HE0006CS

Synonyms

CST3; cystatin C; cystatin-C; cystatin 3; cystatin-3; CYSC; MGC105556;

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Molecular Mass

14.3kDa (128aa)
13.5-18KDa (SDS-PAGE under reducing conditions.)

AA Sequence

GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPHLMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5mg/ml (determined by Absorbance at 280nm)

Warning

For research use only!

Background

Cst3 played an important role in many functions.

References

Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.
Esnard A., et al. (1992) FEBS Lett. 300:131-135.

Tag

His tag

Species

Rat

Source

Insect cells

BACKGROUND

Background

Cst3 played an important role in many functions.

References

Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.
Esnard A., et al. (1992) FEBS Lett. 300:131-135.

Reference Reading

1. Kim, J-T; Lee, S-J; Kang, M. A.; et al. Cystatin SN neutralizes the inhibitory effect of cystatin C on cathepsin B activity, CELL DEATH & DISEASE, 4, e974.

"In this study, we showed that CST1 was highly expressed in colon tumor tissues, compared with nontumor regions. Increased cell proliferation and invasiveness were observed in HCT116 cell lines stably transfected with CST1 cDNA (HCT116-CST1) but not in CST3-transfected cells. We also demonstrated that CST1-overexpressing cell lines exhibited increased tumor growth as well as metastasis in a xenograft nude mouse model. Interestingly, CST1 interacted with cystatin C (CST3), a potent cathepsin B (CTSB) inhibitor, with a higher affinity than the interaction between CST3 and CTSB in the extracellular space of HCT116 cells."