Recombinant Rhesus IL4 Protein (RE0012IL)

Cat.No.

RE0012IL

Synonyms

Interleukin-4; IL4; IL-4; BSF-1;

Accession

IL4

Form

Lyophilized from sterile 2 × PBS, pH 7.4, 5 % trehalose.

Bio-activity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of> 1.0 × 10^6 IU/mg.

Molecular Mass

Recombinant Rhesus IL4 Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 14.9 kDa.

AA Sequence

HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 96 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Dissolve with double distilled water.

Warning

For research use only!

Background

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

Species

Rhesus

Source

E. coli

Background

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.