Recombinant Mouse Podxl Protein(His tag) (MI0004PS)

Recombinant Mouse Podxl Protein (NP_038751) (22-404aa) with C-terminus His tag was expressed in Insect cells.

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PRODUCT INFORMATION

Cat.No.

MI0004PS

Synonyms

PODXL; podocalyxin-like; podocalyxin; PC; Ly102; Pclp1; PCLP-1; AW121214;

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Molecular Mass

41.0kDa (391aa)
70-100KDa (SDS-PAGE under reducing conditions.)

AA Sequence

HNGNETSTSA IKSSTVQSHQ SATTSTEVTT GHPVASTLAS TQPSNPTPFT TSTQSPSMPT STPNPTSNQS GGNLTSSVSE VDKTKTSSPS STAFTSSSGQ TASSGGKSGD SFTTAPTTTL GLINVSSQPT DLNTTSKLLS TPTTDNTTSP QQPVDSSPST ASHPVGQHTP AAVPSSSGST PSTDNSTLTW KPTTHKPLGT SEATQPLTSQ TPGITTLPVS TLQQSMASTV GTTTEEFTHL ISNGTPVAPP GPSTPSPIWA FGNYQLNCEP PIRPDEELLI LNLTRASLCE RSPLDEKEKL VELLCHSVKA SFKPAEDLCT LHVAPILDNQ AVAVKRIIIE TKLSPKAVYE LLKDRWDDLT
EAGVSDMKLG KEGPPEVNED RFSLEHHHHH H

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5mg/ml (determined by Absorbance at 280nm)

Warning

For research use only!

Background

Podxl played an important role in many functions.

References

Vitureira N., et al. (2010) PLoS ONE 8:e12003.
Nielsen JS., et al. (2007) PLoS ONE 2:E237.

Tag

His tag

Species

Mouse

Source

Insect cells

BACKGROUND

Background

Podxl played an important role in many functions.

References

Vitureira N., et al. (2010) PLoS ONE 8:e12003.
Nielsen JS., et al. (2007) PLoS ONE 2:E237.

Reference Reading

1. Horrillo, Angelica; Porras, Gracia; Ayuso, Matilde S.; et al. Loss of endothelial barrier integrity in mice with conditional ablation of podocalyxin (Podxl) in endothelial cells, EUROPEAN JOURNAL OF CELL BIOLOGY, 95-8, 265-276.

"Podocalyxin (Podxl) has an essential role in the development and function of the kidney glomerular filtration barrier. It is also expressed by vascular endothelia but perinatal lethality of podxl(-/-) mice has precluded understanding of its function in adult vascular endothelial cells (ECs). In this work, we show that conditional knockout mice with deletion of Podxl restricted to the vascular endothelium grow normally but most die spontaneously around three months of age."

2. Kang, Lei; Yao, Chunping; Khodadadi-Jamayran, Alireza; et al. The Universal 3D3 Antibody of Human PODXL Is Pluripotent Cytotoxic, and Identifies a Residual Population After Extended Differentiation of Pluripotent Stem Cells, STEM CELLS AND DEVELOPMENT, 25-7, 556-568.

"Podocalyxin-like protein (PODXL) is a member of CD34 family proteins. It is the protein that carries many post-translational epitopes responsible for various pluripotent surface markers including TRA-1-60, TRA-1-81, GCTM2, GP200, and mAb84. However, PODXL has not attracted the attention of stem cell biologists."

3. Gebeshuber, Christoph A.; Kornauth, Christoph; Dong, Lihua; et al. Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1, NATURE MEDICINE, 19-4, 481-+.

"Mechanistically, miR-193a inhibits the expression of the Wilms' tumor protein (WT1), a transcription factor and master regulator of podocyte differentiation and homeostasis. Decreased expression levels of WT1 lead to downregulation of its target genes PODXL (podocalyxin) and NPHS1 (nephrin), as well as several other genes crucial for the architecture of podocytes, initiating a catastrophic collapse of the entire podocyte-stabilizing system."