Recombinant Mouse Pigr Protein(His tag) (MH0229PL)

Recombinant Mouse Pigr Protein (O70570) (Met 1-Lys 645) with a C-terminal polyhistidine tag was expressed in HEK293.

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PRODUCT INFORMATION

Cat.No.

MH0229PL

Synonyms

PIGR; poly-Ig receptor

Accession

Pigr

Predicted N Terminal

Lys 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PIGR at 5 μg/ml (100 μl/well) can bind mouse IgM with a linear range of 0.156-10 μg/ml.

Molecular Mass

Recombinant Mouse PIGR consists of 638 amino acids and has a calculated molecular mass of 70.8 kDa. As a result of glycosylation, Recombinant protein migRates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 97 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment (By similarity).

Tag

His

Species

Mouse

Source

HEK293

BACKGROUND

Background