Recombinant Mouse Pdcd1 Protein(His tag) (MH0230PL)

Recombinant Mouse Pdcd1 Protein (Q02242) (Met 1-Gln 167) with a polyhistidine tag at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

MH0230PL

Synonyms

PDCD1; mPD-1; PD-1; Pdc1

Accession

Pdcd1

Predicted N Terminal

Leu 25

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized mouse PDCD1-His at 10 μg/ml (100 μl/well) can bind mouse CD274-Fch, The EC50 of mouse CD274-Fch is 1.0-2.3μg/ml.

Molecular Mass

Recombinant Mouse PDCD1 consists of 154 amino acids and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm PDCD1 is approximately 40-45 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors (By similarity).

Tag

His

Species

Mouse

Source

HEK293

BACKGROUND

Background