Bio-activity
The ED50, calculated by the dose-dependant proliferation of murine BALB 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.
Storage
Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IGF-1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.