Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.0, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant mouse Hemopexin binds>15 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of mouse Hemopexin.
Molecular Mass
Recombinant Mouse HPX consists of 448 amino acids and has a predicted molecular mass of 50.4 kDa. The apparent molecular mass of the rm HPX is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Tag
His
Species
Mouse
Source
HEK293
BACKGROUND
Background
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
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