Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
Bio-activity
rHirudin is fully biologically active when compared to standard. Its specific activity is> 1×10^4 ATU/mg.
Molecular Mass
Recombinant Medicinal leech Hirudin Protein contains 65 amino acid residues and having a molecular mass of 6979.5 Da.
Endotoxin
< 0.01 EU per 1 microgram of protein (determined by LAL method).
Purity
> 96 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!
Background
Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.