Recombinant Human VEGFC Protein(His tag) (HI0002VS)

Recombinant Human VEGFC Protein (NP_005420) (112-227aa) with C-terminus His tag was expressed in Insect cells.

Size	Price	Qty
50ug	$660.00

Order Online Bulk Inquiry

PRODUCT INFORMATION

Cat.No.

HI0002VS

Synonyms

VEGFC; VRP; VEGF-C; FLT4 ligand DHM; Flt4-L

Accession

VEGFC

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 20% glycerol.

Molecular Mass

14.2kDa (125aa)
18-28KDa (SDS-PAGE under reducing conditions.)

AA Sequence

ADPAHYNTEI LKSIDNEWRK TQCMPREVCI DVGKEFGVAT NTFFKPPCVS VYRCGGCCNS EGLQCMNTST SYLSKTLFEI TVPLSQGPKP VTISFANHTS CRCMSKLDVY RQVHSIIRRH HHHHH

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5mg/ml (determined by Absorbance at 280nm)

Warning

For research use only!

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors.

References

Hatano Y., et al. (2014) Biochem. Biophys. Res. Commun. 446:149-154.
Ciobanu M., et al. (2013) Rom J Morphol. Embryol. 54:925-934.

Tag

His tag

Species

Human

Source

Insect cells

BACKGROUND

Background

References

Hatano Y., et al. (2014) Biochem. Biophys. Res. Commun. 446:149-154.
Ciobanu M., et al. (2013) Rom J Morphol. Embryol. 54:925-934.

Reference Reading

1. Koltowska, Katarzyna; Lagendijk, Anne Karine; Pichol-Thievend, Cathy; et al. Vegfc Regulates Bipotential Precursor Division and Prox1 Expression to Promote Lymphatic Identity in Zebrafish. CELL REPORTS, 13-9, 1828-1841.

"Vegfc drives cell division and Prox1 expression in lymphatic daughter cells, coupling signaling dynamics with daughter cell fate restriction and precursor division."

2. Koltowska, Katarzyna; Paterson, Scott; Bower, Neil I.; et al. mafba is a downstream transcriptional effector of Vegfc signaling essential for embryonic lymphangiogenesis in zebrafish. GENES & DEVELOPMENT, 29-15, 1618-1630.

"Mechanistically, Vegfc signaling increases mafba expression to control downstream transcription, and this regulatory relationship is dependent on the activity of SoxF transcription factors, which are essential for mafba expression in venous endothelium. Here we identify an indispensable Vegfc-SoxF-Mafba pathway in lymphatic development."

3.Jung, Michaela; Oeren, Bilge; Mora, Javier; et al. Lipocalin 2 from macrophages stimulated by tumor cell-derived sphingosine-1-phosphate promotes lymphangiogenesis and tumor metastasis, SCIENCE SIGNALING, 9-434.

"Conditioned medium from coculture experiments indicated that the release of LCN2 from macrophages induced tube formation and proliferation in cultures of primary human lymphatic endothelial cells in amanner dependent on the kinase PI3K and subsequent induction of the growth factor VEGFC, which functioned as an autocrine signal stimulating the receptor VEGFR3."