Recombinant Human TEK Protein(Fc-His tag) (HH0142TL)

Recombinant Human TEK Protein (Q02763) (Met 1-Lys 745) with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0142TL

Synonyms

TEK; CD202b; TIE 2; TIE2; VMCM1; hTIE2; VMCM; TIE-2; CD202B;

Accession

TEK

Predicted N Terminal

Ala 23

Form

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.

Molecular Mass

Recombinant Human Tie2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and predicts a molecular mass of 108.5 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2/Fc monomer is approximately 125-135 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Tag

Fc-His

Species

Human

Source

HEK293

BACKGROUND

Background